Direct measurement of sialic acids from glycoprotein hydrolysis by HPLC-CAD

Instrument Type: HPLC

A isocratic method was developed for determine sialic acids content in glycoprotein using Thermo Scientific U3000 HPLC system with charged aerosol detector. Sialic acids were released from human and bovine transferrin with enzyme digestion and seperated on a Dionex Trinity P2 column without derivatization. N-acetylneuraminic acid and N-glycolyneuraminic acid can be determined in less than 12 min with this method. Sample solvent: 40% 100mM ammonium acteate at pH5, 60% acetonitrile