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USP 38 monograph: impurity determination of trimethoprim using a C18 HPLC column
Instrument Type: HPLCThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of trimethoprim and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the USP 38 monograph. The results obtained for resolution exceeded the criteria stated in the USP. Resolution between trimethoprim and diaveridine peak was 2.97 (USP criteria of not less than 2.5).
