Quantitative Analysis of Ractopamine in Beef using Automated Online Sample Preparation with Liquid Chromatography-Tandem Mass Spectrometry

Instrument Type: LCMSMS

Ractopamine is a phenethanolamine member of the family of b2-adrenergic receptor agonists (b-agonists). It has been widely used as a veterinary additive drug in livestock production to promote leanness in meat, accelerate average daily weight gain and improve feed efficiency. Recently, there have been growing concerns about the safety of meat containing ractopamine residues due to its potential health risks for humans. We describe a rapid and sensitive automated online sample preparation LC-MS/MS method to determine ractopamine in beef.

Study of β-agonist Residues in Animal-derived Foods by LC-MS/MS

Instrument Type: LCMSMS

We developed a sensitive and reproducible LC-MS/MS method to detect ten β-agonists drugs in animal-derived foods.

Effective extraction of beta-agonists from pork using in-line SPE

Instrument Type: HPLC

In this example, pork was extracted with organic solvents in a Thermo Scientific ASE Accelerated Solvent Extractor. Evaporation and reconstitution were difficult, so it was overcame by using solid-phase extraction (SPE). SPE is most effective when it is orthogonal to the analytical separation, so a Thermo Scientific Acclaim Mixed-Mode WCX-1 cartridge was used to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.