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Fast analysis of glutamate in beer by automated precolumn OPA derivatization

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of glutamate in beer. The sample preparation is automated, and the separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 330 nm. Chromatogram A is beer and chromatogram B is beer spiked with 250 µg/mL glutamic acid.