AN106: Ion Chromatography in the Pharmaceutical Industry (Mixed-Mode Column with UV Detection).

Instrument Type: HPLC

Ion Chromatography is a simple and accurate technique for the analysis and quantification of charged species. This application note describes the use of ion exchange chromatography and ion exclusion chromatography in the pharmaceutical industry for the analysis of ions in ingredients and final products. The topic of cleaning validation is also discussed as it applies to charged species. Here shows the separation of a series of aromatic acids by using an anion exchange column (OmniPac PAX-100) and UV absorbance detection.