LC-MS quantitative screening method for 18 anabolic steroids in oral fluid using MS2 spectra data collected with Q Exactive Orbitrap mass spectrometer

Instrument Type: LCMSMS

Androgenic-anabolic steroids (AAS) are drugs which mimic effects of testosterone and dihydrotestosterone in the human body. They increase protein synthesis within cells which results in buildup of cellular tissue, especially in muscles. Use of anabolic steroids by athletes to increase body weight is referred to as doping and is banned by major sporting bodies. In this work we implemented Thermo Scientific Q Exactive ultra high resolution mass spectrometer to ensure high method specificity and sensitivity.