The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of cationic, nonionic, amphoteric and anionic surfactants. The separation is performed using a Thermo Scientific Acclaim Surfactant Plus column, with charged aerosol detection. The Thermo Scientific Acclaim Surfactant Plus column, based on novel mixed mode chromatography technology and advanced surface chemistry, provides both reversed-phase and anion-exchange retention mechanisms.