Red wine is a rich source of phenolic antioxidants and is reported to reduce the risk of diabetes, cancer, Alzheimer's disease, and cardiovascular disease. To improve grape yields it is common practice in vineyards to use pesticides, such as fungicides and insecticides. This application presents a fast, easy, and cost-effective method for the determination of 24 pesticide residues in red wine using the QuEChERS extraction method (AOAC version), cleanup by dispersive solid-phase extraction (dSPE), followed by LC-MSMS analysis.