The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Lorazepam and its Organic impurities as per its USP method. The analysis was performed on a Thermo Scientific Syncronis C18 L1 HPLC column using the method described in the USP 39 monograph. The results obtained were found to be within acceptance criteria as stated in the USP. Resolution between Lorazepam Rel Comp A and Lorazepam Rel Comp E was 2.55 (USP criteria of not less than 2.0). Tailing Factor for Lorazepam standard solution was found to be 0.95 (USP criteria of not more than 2.0).
