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AN74042: Structural Characterization of Mucin O-linked Glycans using a Novel Anion Exchange Column in HPAE-PAD-MS
Description
In this application note, we demonstrate a workflow combining the chemical release of O-linked glycans, followed by sample cleanup and analysis by HPAE-PAD hyphenated to a Q Exactive HF mass spectrometer. The Dionex CarboPac PA300-4μm column uses a novel column technology that enables the simultaneous separation of neutral and charged glycans without the need of derivatization. High resolution accurate mass MS data and tandem MS/MS spectra with diagnostic fragment ions provide highly reliable structural annotations of heterogenous glycans.| Market: | BioPharma |
| Keywords: | oligosaccharide, HPAE-PAD, Orbitrap, HRMS, TN 20, TN 70671, TN20, TN70671, mucin, AN 74042, HPAE-PAD/MS, 0-linked oligosaccharide, AN74042, HRAMS |
| Matrix: | Beta-elimination released oligosaccharides |
| Author: | Tian Tian, Neil Rumachik, Yuanxue Hou, Jun Cheng, Charanjit Saini, Yan Liu, Chris Pohl |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
