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AN000472: HPAE-PAD Method for Determination of Hib Capsular Polysaccharide Content Be the first to rate this application


This work describes an HPAE-PAD method for the determination of ribitol in Hib capsular polysaccharide acid hydrolysis samples. Separation of ribitol from TFA acid hydrolysis products was achieved under isocratic elution conditions using a Thermo Scientific™ Dionex™ CarboPac™ MA1 column. The method proposed here was validated for performance with respect to linearity, precision, sensitivity, and accuracy. The acid hydrolysis samples were also tested using a palladium hydrogen reference electrode.
Market: BioPharma
Keywords: Dionex CarboPac MA1 Column, Dionex ICS-5000+ System, vaccine, TN71, TN 71, Dionex ICS-6000 system, TN 20, TN 70671, TN20, TN70671, Haemophilus influenzae, ribitol, palladium hydrogen reference electrode, AN 000472
Matrix: DI water
Author: Sachin Patil and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA, USA
Uploaded on 12/28/2021.

For Research Use Only. Not for use in diagnostic procedures.