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AN000685: Determination of KDO from Bacterial Polysaccharides Be the first to rate this application

Description

KDO (3-deoxy-D-manno-oct-2-ulosonic acid) is an eight-carbon sugar mostly confined to Gram-negative bacteria. KDO can be used to quantify the amount of a known lipopolysaccharide (LPS) (the moles of KDO per mole of liposaccharide must be known). The hydrolysis conditions were optimized for the release of KDO from an LPS and a method for its quantitation has been developed using HPAE-PAD. In this application note, we develop, optimize, and validate a HPAE-PAD method for the determination of KDO.
Market: BioPharma
Keywords: HPAE-PAD, TN 20, TN 70671, TN20, TN70671, endotoxins, ICS6000, Lipopolysaccharides, LPS, bacterial polysaccharides, CarboPac PA20-Fast-4 µM column, AN 000685
Matrix: Lipopolysaccharides
Author: Manali Aggrawal and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 10/4/2021.

For Research Use Only. Not for use in diagnostic procedures.