Thermo Scientific AppsLab Library: AN000685: Determination of KDO from Bacterial Polysaccharides

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Description

KDO (3-deoxy-D-manno-oct-2-ulosonic acid) is an eight-carbon sugar mostly confined to Gram-negative bacteria. KDO can be used to quantify the amount of a known lipopolysaccharide (LPS) (the moles of KDO per mole of liposaccharide must be known). The hydrolysis conditions were optimized for the release of KDO from an LPS and a method for its quantitation has been developed using HPAE-PAD. In this application note, we develop, optimize, and validate a HPAE-PAD method for the determination of KDO.

Market:	BioPharma
Keywords:	HPAE-PAD, TN 20, TN 70671, TN20, TN70671, endotoxins, ICS6000, Lipopolysaccharides, LPS, bacterial polysaccharides, CarboPac PA20-Fast-4 µM column, AN 000685
Matrix:	Lipopolysaccharides
Author:	Manali Aggrawal and Jeffrey Rohrer
Affiliation:	Thermo Fisher Scientific, Sunnyvale, CA USA

Uploaded on 10/4/2021.

For Research Use Only. Not for use in diagnostic procedures.