CAN73265: Targeted Metabolomics Method using IC Orbitrap with High Resolution Mass Spectrometry
DescriptionA number of different water-soluble compound standards were used, and the retention time and MS/MS spectra for more than 350 types of compounds that can be analyzed with this system were acquired to construct a metabolite library. Additionally, using cultured animal cells and human serum samples, optimization of pretreatment for the IC Q Exactive system was studied. Using the IC Q Exactive system, the conditions for IC separation were optimized, and MS and MS/MS spectral libraries were constructed.
|Keywords:||Amino acids, Nucleotides, Sugar phosphates, Organic acids, IonPac AS11-HC, metabolomics, cell extracts, CAN 73265|
|Matrix:||Blood, Cell extracts from HeLa cells, Serum|
|Author:||Yoshihiro Izumi and Takeshi Bamba|
|Affiliation:||Kyushu University, Research Center for Transomics Medicine, Division of Metabolomics|
For Research Use Only. Not for use in diagnostic procedures.