Thermo Scientific AppsLab Library: AN000068: O-Glycan Profiling using HPAE-PAD Hyphenated with a High-Resolution Accurate Mass (HRAM) Mass Spectrometer

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AN000068: O-Glycan Profiling using HPAE-PAD Hyphenated with a High-Resolution Accurate Mass (HRAM) Mass Spectrometer Be the first to rate this application

Description

We demonstrate a workflow combining chemical release of O-linked glycans, followed by sample cleanup and analysis by HPAE-PAD hyphenated to a Q Exactive HF Hybrid Quadrupole-Orbitrap MS. A recently introduced Dionex CarboPac PA300-4μm column, enables simultaneous separation of neutral and charged glycans without the need for derivatization. High-resolution MS data and MS/MS spectra with diagnostic fragment ions provide reliable structural annotations of heterogeneous glycans. We applied HPAE-PAD/MS to analyze O-glycan structures released from four different glycoproteins.

Market:	BioPharma
Keywords:	Dionex ICS-5000+, Glycans, Glycoproteins, Glycosylation, Pulsed Amperometric Detection, Bovine fetuin, HPAE-PAD, Q Exactive, Orbitrap, TN71, TN 20, TN 70671, TN20, TN70671, Dionex ICS-6000, bovine fibrinogen, bovine thyroglobulin, Dionex CarboPac PA300 column, mucin, AN 000068
Matrix:	Glycoproteins
Author:	Manali Aggrawal; Sachin Patil; Jeffrey Rohrer
Affiliation:	Thermo Fisher Scientific, Sunnyvale, CA USA

Uploaded on 7/26/2021.

For Research Use Only. Not for use in diagnostic procedures.