Thermo Scientific AppsLab Library: Evaluation of custom injection programs and larger internal diameter capillary for strong solvent sample effects mitigation in liquid chromatography

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Fig. 1. Effect of the sample strong solvent on the 3 mm ID column compared to sample in mobile phase.

Fig. 2. Effect of the sample strong solvent on the 4.6 mm ID column compared to sample in mobile phase.

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Description

This technical note describes two of the mentioned strategies to overcome or reduce the SSE, namely the Strong Solvent Loop (SSL) and CIPs. Both approaches can deliver satisfying chromatographic results with limited effort or system adaptation. The SSE is shown and discussed at five injection levels, and the mitigation of SSE by implementing SSL and CIPs is evaluated for two column dimensions and three different sample solvents.

Market:	Other
Keywords:	asymmetry, peak results, peak width, Vanquish Core HPLC system, custom injection program, strong solvent loop, strong solvent samples, dispersion, peak shape
Matrix:	Acetonitrile, dimethyl sulfoxide, Methanol
Author:	Denis Andrei Ispan, Tibor Müllner, Maria Grübner
Affiliation:	Thermo Fisher Scientific

Uploaded on 6/11/2021.

For Research Use Only. Not for use in diagnostic procedures.