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AN71993: Profiling Galactosyloligosaccharide-Containing Samples by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Be the first to rate this application


GOS occur naturally in the milk of many animals including humans, cows, and wallabies. GOS are primarily composed of galactose and often terminate with a glucose residue at the reducing end. These carbohydrates are enzymatically produced by transgalactosylation reactions of lactose catalyzed by β-galactosidases and give rise to galactose oligomers. Separation of individual oligosaccharides in GOS was achieved on a Thermo Scientific™ Dionex™ CarboPac™ PA200 column. Oligosaccharide detection was by PAD with a gold working electrode, and therefore no sample derivation was required.
Market: Food and Beverage
Keywords: Carbohydrates, GOS, oligosaccharide, Infant Formula, prebiotics, probiotics, TN21, TN71, Dionex CarboPac PA200, disposable electrode, AN 71993
Matrix: Infant formula
Author: Manali Aggrawal; Jeff Rohrer
Affiliation: Thermo Fisher Scientific
Uploaded on 12/12/2019.

For Research Use Only. Not for use in diagnostic procedures.