Thermo Scientific AppsLab Library: AN71993: Profiling Galactosyloligosaccharide-Containing Samples by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

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AN71993: Profiling Galactosyloligosaccharide-Containing Samples by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Be the first to rate this application

Description

GOS occur naturally in the milk of many animals including humans, cows, and wallabies. GOS are primarily composed of galactose and often terminate with a glucose residue at the reducing end. These carbohydrates are enzymatically produced by transgalactosylation reactions of lactose catalyzed by β-galactosidases and give rise to galactose oligomers. Separation of individual oligosaccharides in GOS was achieved on a Dionex™ CarboPac™ PA200 column. Oligosaccharide detection was by PAD with a gold working electrode, and therefore no sample derivation was required. This replaced AN1151.

Market:	Food and Beverage
Keywords:	Carbohydrates, GOS, oligosaccharide, Infant Formula, prebiotics, probiotics, TN21, TN71, Dionex CarboPac PA200, disposable electrode, AN 71993, AN 1151, AN1151, TN 20, TN 70671, TN20, TN70671
Matrix:	Infant formula
Author:	Manali Aggrawal; Jeff Rohrer
Affiliation:	Thermo Fisher Scientific

Uploaded on 12/12/2019.

For Research Use Only. Not for use in diagnostic procedures.