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Fast and Reproducible peptide mapping of an Fusion Protein (Aflibercept) by using a SMART digest Kit and solid core C18 column. Be the first to rate this application


The Thermo Scientific Dionex Ultimate 3000 system is applied for the peptide mapping analysis of an Fusion Protein (Aflibercept). The separation was performed on a Thermo Scientific Accucore vanquish C18+ column by using a Thermo Scientific SMART digest kit at UV detection of 210 nm. The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.
Market: BioPharma
Keywords: Peptide mapping, UV detection, UHPLC, Trypsin digestion, SMART Digest kit, Fusion protein, Aflibercept
Matrix: NA
Author: Ankitkumar Patel
Affiliation: Thermo Fisher Scientific, CoE Lab, Ahmedabad, India
Uploaded on 11/25/2019.