Fast and Reproducible peptide mapping of an Fusion Protein (Aflibercept) by using a SMART digest Kit and solid core C18 column.
DescriptionThe Thermo Scientific Dionex Ultimate 3000 system is applied for the peptide mapping analysis of an Fusion Protein (Aflibercept). The separation was performed on a Thermo Scientific Accucore vanquish C18+ column by using a Thermo Scientific SMART digest kit at UV detection of 210 nm. The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.
|Keywords:||Peptide mapping, UV detection, UHPLC, Trypsin digestion, SMART Digest kit, Fusion protein, Aflibercept|
|Affiliation:||Thermo Fisher Scientific, CoE Lab, Ahmedabad, India|