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Online 2D-nanoLC hyphenated with high-resolution accurate-mass Orbitrap mass spectrometry for comprehensive and robust proteome profiling
Description
In this technical note, we developed and elucidated the benefits of an online 2D nanoLC-MS platform for proteomics. We present a simple-to-use online low-flow high-pH RP x low-pH RP separation platform (termed online 2D-nanoLC) for deep proteome profiling using a Thermo Scientific™ UltiMate™ 3000 RSLCnano system coupled to a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer. This automated approach not only reduces sample complexity but also boosts peptide/protein identifications and quantification without extra sample handling steps.| Market: | Proteomics |
| Keywords: | Proteomics, Q Exactive HF-X, nanoLCMS, UltiMate 3000 RSLCnano, Orbitrap Exploris 480, Deep Proteome Profiling, online 2D-LC |
| Matrix: | HeLa Cell Lysate Protein Digest, Serum Protein Digest |
| Author: | Runsheng Zheng, Alexander Boychenko, Tabiwang N. Arrey, Christopher Pynn, Wim Decrop, Peter Jehle |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany; Thermo Fisher Scientific, Bremen, Germany |
| Status: | Proof of Concept |
For Research Use Only. Not for use in diagnostic procedures.
