Thermo Scientific AppsLab Library: Robust extraction, separation, and quantitation of structural isomer steroids from human plasma by SPE-UHPLC-MS/MS

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Separation of common steroids of interest with method 1

Chromatogram showing separation of components with method 2

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Description

The Thermo Scientific Accucore Biphenyl column offers alternative selectivity to a C18 column for clinical research. This high-resolution column separates isomers and steroids utilizing superior combination of hydrophobic, aromatic, and polar selectivity.

Market:	Clinical Research
Keywords:	LC-MS/MS, SPE, Steroid hormone, TSQ Quantiva, Accucore Biphenyl column, Vanquish Horizon UHPLC
Matrix:	Human plasma
Author:	Kean Woodmansey, Jon Bardsley, Stacy Tremintin
Affiliation:	Thermo Fisher Scientific

Uploaded on 5/22/2019.

For Research Use Only. Not for use in diagnostic procedures.