AU72829: HPAE-PAD Analysis of N-linked Glycans: Improving Glycan Resolution
DescriptionThis application update demonstrates improved resolution of sialylated N-glycans on Thermo Scientific™ Dionex™ CarboPac™ PA200 columns. Starting with a recently described method, changes to elution conditions were tested to improve resolution of sialylated N-glycans enzymatically released from four different glycoproteins. Separations were first evaluated on the analytical format (3 × 250 mm column). Next, the possibility that a shorter column, such as a guard column, would allow significantly improved throughput was evaluated. Finally, a narrow bore format 1 × 250 mm column was tested.
|Keywords:||Electrochemical detection, oligosaccharide, HPAE-PAD, Biopharmaceuticals, CarboPac PA200 column, Protein glycosylation, Sialylated glycans, TN 20, TN 70671, TN20, TN70671, AU 72829|
|Author:||Sachin Patil and Jeff Rohrer|
|Affiliation:||Thermo Fisher Scientific Sunnyvale, CA|
For Research Use Only. Not for use in diagnostic procedures.