Thermo Scientific AppsLab Library: AU72829: HPAE-PAD Analysis of N-linked Glycans: Improving Glycan Resolution

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Description

This application update demonstrates improved resolution of sialylated N-glycans on Thermo Scientific™ Dionex™ CarboPac™ PA200 columns. Starting with a recently described method, changes to elution conditions were tested to improve resolution of sialylated N-glycans enzymatically released from four different glycoproteins. Separations were first evaluated on the analytical format (3 × 250 mm column). Next, the possibility that a shorter column, such as a guard column, would allow significantly improved throughput was evaluated. Finally, a narrow bore format 1 × 250 mm column was tested.

Market:	BioPharma
Keywords:	Electrochemical detection, oligosaccharide, HPAE-PAD, Biopharmaceuticals, CarboPac PA200 column, Protein glycosylation, Sialylated glycans, TN 20, TN 70671, TN20, TN70671, AU 72829
Matrix:	DI water
Author:	Sachin Patil and Jeff Rohrer
Affiliation:	Thermo Fisher Scientific Sunnyvale, CA

Uploaded on 10/15/2018.

For Research Use Only. Not for use in diagnostic procedures.