Rapid quantitation of therapeutic antibodies in animal plasma
DescriptionTo demonstrate the rapid quantitation of a low-level human IgG in animal plasma by LC/MS/MS using the Thermo Scientific™ SMART Digest™ Immunoaffinity (IA) kits (including magnetic and non-magnetic versions of the Streptavidin kit, Protein A kit, and Protein G kit), which combine the IA capture and digestion process into a single well. The assay is required to be both selective and accurate.
|Keywords:||Human IgG, Biopharmaceutical, SRM, UHPLC, IgG, Quantitation, MRM, Protein A, Sample preparation, Biotherapeutic, 96 well plates, Accucore™ C18, affinity proteomics, bioanalysis, biotin, immunoaffinity, immunocapture, monoclonal antibody (mAb), peptide analysis, protein digestion, protein enzyme digestion, Protein G, protein magnetic beads, SMART Digest™ Immunoaffinity (IA) Kits, Streptavidin|
|Author:||Xin Zhang1 , John O’Grady2 , Kevin Meyer2|
|Affiliation:||1. Thermo Fisher Scientific, Sunnyvale, CA, USA 2. Perfinity Biosciences Inc., West Lafayette, IN, USA|
For Research Use Only. Not for use in diagnostic procedures.