Find methods for your needs

AN72780: Determination of Lactose in Lactose-Free Dairy Products using HPAE Coupled with PAD and MS Dual Detection Be the first to rate this application


In this application, we used mass spectrometry (MS) along with pulsed amperometric detection (PAD) to detect residual lactose in lactose-free dairy products. A Dionex™ CarboPac™ PA210-4μm column separated lactose from structurally similar milk sugars using KOH eluent produced online by an eluent generator. A “tee” was placed immediately after the column to split the flow, thus enabling both PAD and MS detection. Coupling MS to the HPAE separation provides higher detection selectivity and sensitivity for this application.
Market: Food and Beverage
Keywords: Dionex ICS-5000+, Lactulose, Pulsed Amperometric Detection, Lactose, HPAE-PAD, dairy products, Epilactose, allolactose, Lactose-free, Dionex ICS-6000 system, Dionex CarboPac PA210-4μm column, ISQ EC single quadrupole mass spectrometer, lactose-free butter, lactose-free dairy products, lactose-free milk, lactose-free yogurt, low-lactose dairy products
Matrix: Low lactose/lactose-free dairy products
Author: Manali Aggrawal; Jeff Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale CA
Uploaded on 8/27/2018.

For Research Use Only. Not for use in diagnostic procedures.