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High-throughput capillary-flow LC-MS proteomics with maximum MS utilization
Description
Here we describe an example of our high throughput proteomics applications. Using the UltiMate 3000 RSLCnano system, the presented intelligent method setup affords a total cycle time of only 8 min with a 6 min peptide elution window by employing advanced washing steps that are executed in parallel with a high speed (1.5µL/min) separation on a nano (75µm I.D.) column. The method is fully customizable and can be altered in seconds to increase the peptide elution window by 2, 5, 10, … minutes according to the sample complexity or depth of proteome coverage you require.| Market: | Proteomics |
| Keywords: | nanoLC, EASY-Spray, High-throughput low-flow LC, LC-MS based proteomics, Pre-concentration setup, Q Exactive HF-X, RSLCnano |
| Matrix: | cell digest, protein digest, proteome |
| Author: | Oleksandr Boychenko, Christopher Pynn, Bart van den Berg, Tabiwang N. Arrey, Wim Decrop, Martin Ruehl |
| Affiliation: | Thermo Fisher Scientific, Germering, Germany |
For Research Use Only. Not for use in diagnostic procedures.
