AN72580: An Improved HPAE-PAD Method for Glycoprotein Monosaccharide Determination
DescriptionThis work demonstrates an improved HPAE-PAD method using a recently introduced Thermo Scientific™ Dionex™ CarboPac™ PA20 column with 4 μm particle size to simultaneously quantify six major monosaccharides present in glycoprotein acid digests. The total run time is 20 min as compared to 32 min with the method using a 6.5 μm particle size column. The smaller particle size of this column offers higher peak efficiencies, leading to high-resolution separations and allowing a shorter column format and, ultimately, significantly shorter run times. An ICS-6000 can be used for this application.
|Keywords:||Carbohydrates, Dionex ICS-5000+, Electrochemical detection, fetuin, Biopharmaceutical, PAD, RFIC System, alpha- 1-acid glycoprotein, Dionex CarboPac PA20-Fast-4μm column, ICS-6000|
|Author:||Sachin Patil and Jeffrey Rohrer|
|Affiliation:||Thermo Fisher Scientific, Sunnyvale, CA, USA|
For Research Use Only. Not for use in diagnostic procedures.