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TN72478: Instrument Configuration for Native N-linked Oligosaccharide Characterization by HPAE-PAD/MS - Gradient 3 Be the first to rate this application

native oligosaccharides analyzed by hpaepadms


The biopharmaceutical industry requires reliable high throughput analysis of N-linked glycans. Many of the commonly used analytical approaches are based on derivatization, which is known to introduce differential loss of sialic acid and incomplete labeling. Preparation of native glycans is not subject to these concerns. This approach starts with a fast in-solution glycoprotein digestion to obtain oligosaccharides. These native glycans are then analyzed by high-performance anion-exchange chromatography with pulsed amperometric detection coupled to MS. This record is for neutral and IgG glycans.
Market: BioPharma
Keywords: Carbohydrates, Electrochemical detection, Glycans, glycoprotein, asparagine-linked, PAD, IC-MS, MS/MS, HPAE-PAD, Orbitrap, Glycan, complex oligosaccharides, TN 20, TN 70671, TN20, TN70671, TN 72478
Matrix: glycoprotein oligosaccharides
Author: Zoltan Szabo, J.R. Thayer, and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific, Sunnyvale, CA USA
Uploaded on 11/22/2017.

For Research Use Only. Not for use in diagnostic procedures.