Pharmacopeial assay and organic impurities analysis of Minocycline hydrochloride using a C18 HPLC column
DescriptionThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for assay and organic impurities analysis of minocycline hydrochloride. The analysis was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the USP 39 monograph. The results obtained for resolution and tailing factor met the criteria stated in the USP 39. The results obtained for resolution between epiminocycline and minocycline was 7.5 (USP criteria of NLT 4.6) and tailing factor of minocycline (0.5 mg/mL) was 0.94 (USP criteria of 0.9 to 2.0).
|Keywords:||Acclaim 120 C18 Column, HPLC, UV detection, USP 39 monograph, minocycline, tetracycline antibiotic|
|Affiliation:||Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India|
For Research Use Only. Not for use in diagnostic procedures.