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EP 8.0 monograph: impurity determination of caffeine using a C18 HPLC column
Description
The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Caffeine and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the EP 8.0 monograph. The results obtained for resolution and tailing factor exceeded the criteria stated in the EP. Resolution between impurity C and D was 4.4 (EP criteria of not less than 2.5), resolution between impurity F and A was 4.2 (EP criteria of not less than 2.5), and tailing factor for caffeine was 1.1 (EP criteria not more than 2.0).| Market: | Pharma |
| Keywords: | Acclaim 120 C18, Caffeine, HPLC, UV detection, EP 8.0 monograph |
| Matrix: | N/A |
| Author: | Manish Singh |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
