Two oral, over-the-counter medications were evaluated as representative pharmaceutical products. A cough suppressant and a multisymptom cold/flu medication were chosen because they contain a complex mixture of simple sugars, glycols,and sugar alcohols. These carbohydrates and glycols are also commonly found in other medications. This method uses the CarboPac MA1 column to retain and separate the target analytes.

This application note describes the use of three different anion-exchange columns with amperometric detection to analyze common simple sugars, sugar alcohols, and glycols in pharmaceutical formulations. This record describes a method using the CarboPac PA10 column to determine some of the target carbohydrates. The reader is advised to review TN21 for updated waveform information.

This application note describes the use of three different anion-exchange columns with amperometric detection to analyze common simple sugars, sugar alcohols, and glycols in pharmaceutical formulations. The method in this entry shows how to determine glycols using ion-exclusion chromatography on Dionex ICE-AS1 column.

Glucosamine (GlcN), a naturally occurring amino sugar, is a major structural component in the biosynthesis of glycosaminoglycans. Use of GlcN as a dietary supplement in the management of osteoarthritis has attracted considerable attention. Pulsed amperometric detection (PAD) is sensitive and selective and therefore ideally suited for determination of GlcN. High-performance anion-exchange with pulsed amperometric detection (HPAE-PAD) chromatography is a sensitive, direct-detection technique capable of separating mono- and disaccharides rapidly and efficiently.

Fermentation has been used for centuries to produce alcoholic beverages, bread, cheese, yogurt, and feed stock for animals. To optimize growth and yields, it is crucial to monitor fermentation broths for both cellular fuel sources as well as metabolic byproducts. Alcohols and glycols have been determined using ion exclusion chromatography (ICE) with a Thermo Scientific Dionex IonPac ICE-AS1, using MSA. The waveform and platinum electrode used here allow fast, accurate determinations of alcohols and glycols in fermentation media and alcoholic beverage samples through ICE with PAD.