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Displaying 1-5 of 5 results for: glycan

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Label-Free Profiling of mAb O-linked Glycans by HPLC with Charged Aerosol Detection

Instrument Type: HPLC-CAD

O-glycans released by reductive ß-elimination to preserve structural integrity can be quantitatively measured by a simple direct method that minimizes sample manipulation and cleanup. Released glycans are separated with high resolution by charge, size and polarity on a mixed mode column employing both ion exchange and HILIC separation modes, using a simple, mass spectrometry compatible mobile phase.<enter final 90 characters of description>

Glycans by UHPLC-CAD on Thermo Scientific Dionex UltiMate 3000 RSLC

Instrument Type: HPLC-CAD

Glycans released from bovine fetuin by PNGase-F are separated by gradient elution on the Thermo Scientific Dionex GlycanPac AXR-1 column and detected by charged aerosol detection on the Corona Veo RS.

Fast analysis of sugar phosphates by HPLC-CAD

Instrument Type: HPLC-CAD

The Thermo Scientific UltiMate 3000RS system is applied for the analysis of sugar phosphates. The separation is performed using a Thermo Scientific GlycanPac AXH-1 1.9µm column, with charged aerosol detection. The Thermo Scientific GlycanPac AXH-1 is a column designed to classify anionic carbohydrates by charge and resolve them by HILIC interactions. For this group of simple sugar phosphates, an isocratic analysis is sufficient using a volatile mobile phase that is compatible with charged aerosol detection or mass spectrometry.

Excellent separation of human IgG glycans on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column

Instrument Type: HPLC

Human IgG glycans were separated on a Thermo Scientific Accucore 150-Amide-HILIC HPLC column using 2-AB derivatization and fluorescence detection.

A simple HILIC method for the analysis of bovine fetuin glycans using a Thermo Scientific Accucore 150-Amide-HILIC HPLC column

Instrument Type: HPLC

The analysis of 2AB labelled bovine fetuin glycans was performed on the Thermo Scientific Accucore 150-Amide-HILIC HPLC column. The analysis was simple and robust, leading to the separation and detection of eight individual glycan peaks.