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Displaying 1-5 of 5 results for: peptide mapping

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Fast and Reproducible peptide mapping of an Antibody Drug Conjugate (Kadcyla) by using a SMART digest Kit

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex Ultimate 3000 Bio-LC system is applied for the peptide mapping analysis of an Antibody Drug Conjugate (Kadcyla). The separation was performed on a Thermo Scientific Acclaim RSLC 120-C18 column by using a Thermo Scientific SMART digest kit at UV detection of 214 nm. With a run time of 55 minutes the method allows separation of all peptides of Kadcyla (Antibody Drug Conjugate). The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.

Long-term stable peptide mapping using the Vanquish UHPLCsystem

Instrument Type: UHPLC

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on a Thermo Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions as well as peak capacities were achieved over a time period of more than 80 injections.

Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System

Instrument Type: UHPLC

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.

Column Temperature Control in Peptide Mapping

Instrument Type: UHPLC

The Thermo Scientific Vanquish UHPLC system was used in combination with the Thermo Scientific Acclaim RSLC 120 C18, 2.2 µm, 2.1x250 mm column to provide high resolution peptide separation of a monoclonal antibody digest. The column was thermostatted at 40, 60, and 80 °C. Both still-air and forced-air mode were used. The highest peak capacity was obtained at 80 °C, and with still-air thermostatting mode. At 40 °C, the peak capacity obtained with still-air and forced-air was comparable.

High Resolution Peptide Mapping for Biopharmaceutical Analysis

Instrument Type: HPLC (Biocompatible)

A sample of a monoclonal antibody, was separated by a Thermo Scientific UltiMate 3000 BioRS Dual System. The Thermo Scientific Acclaim RSLC 120 C18, 2.2 µm, 2.1x250 mm column was operated at 80 °C, to obtain high resolution separation of the complex mixture. Due to the high temperature in the column, the pre-heating of the mobile phase was essential to avoid thermal mismatch between the mobile and the column. A pre-column heater 2 µL biocompatiblewas used to achieve thermal equilibration between column and mobile phase.