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Analyte adhesion to LC vials: impact of surface chemistry on recovery at low concentrations
Description
This white paper investigates the impact of vial surface chemistry on analyte recovery in LC and LC-MS workflows, with a focus on adsorption-sensitive biomolecules at low concentrations. It compares different vial materials using representative analytes—including peptides, proteins and monoclonal antibodies—to evaluate how nonspecific binding affects recovery and reproducibility. The study demonstrates that selecting an appropriate vial surface can significantly reduce analyte loss and improve confidence in results, providing practical guidance for optimizing sample handling in routine work.| Market: | BioPharma |
| Keywords: | Peptides, Proteins, Monoclonal Antibodies, GLP-1, RP-HPLC, Adsorbance, Cationic Peptides |
| Matrix: | 0.1% FA in water, 0.1% TFA in water, 20% ACN, Water |
| Author: | Audrius Dorofėjus |
| Affiliation: | Thermo Fisher Scientific, Vilnius, Lithuania |
For Research Use Only. Not for use in diagnostic procedures.
