Drinking water perfluorinated and polyfluorinated alkyl substances (PFAS) studies have typically targeted PFOS and PFBA and other PFAS. Here we discuss the development of an analytical method using an automated SPE system and LC-MS/MS for determination of twenty-five PFAS compounds following the guidelines provided by U.S. EPA Method 533. We have demonstrated that the automated SPE system provides reliable determination of PFAS in large-volume aqueous samples and saves time, solvent, and labor, while ensuring high reproducibility and productivity for analytical testing laboratories.
PFAS workflows are continuously being developed to meet evolving testing requirements for per- and polyfluoroalkyl substances (PFAS) worldwide. This work describes that workflow by leveraging the Dionex AutoTrace 280 PFAS SPE instrument to automate the soil sample solid-phase extraction (SPE) cleanup stage that is described in EPA Method 1633.
PFAS are per- and polyfluoroalkyl substances. The chemical nature of the C-F bonds makes these compounds extremely stable. Hence, PFAS have been given the term “forever compounds”. This application note will present data for measuring 40 PFAS in fortified water samples following the third draft of EPA Method 1633. An MDL study was conducted in reagent water to demonstrate that equivalent or better performance can be attained using the Thermo Scientific™ Vanquish™ Flex Binary UHPLC system and Thermo Scientific™ TSQ™ Quantis Plus mass spectrometer.
The Thermo Scientific UltiMate 3000 RS HPLC System is applied for the chiral purity of (S)-Isomer in Vilanterol Trifenatate. The analysis was performed on a Thermo Scientific Hypersil Chiral IGT HPLC column using an in-house method. The result obtained for resolution between S-isomer and Vilanterol Trifenatate is 4.12.The results obtained for system suitability were 2.37 for the S-isomer and Vilanterol Trifenatate were 1.0 and 1.02 respectively.
