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Tandem NanoLC-MS: Maximum MS Utilization for Deep Dive Proteomic Analysis
Description
Here we show that with a modest hardware extension to the UltiMate 3000 RSLCnano platform, namely the addition of a second nano-flow high-pressure gradient pump and 1/32” nano post column switching valve plus the associated additional fluidics, columns and column switching valve, all of which are commercially available, a tandem nanoLC stack can be created which enables up to twice the throughput capacity compared to a single LC-MS system.| Market: | Proteomics |
| Keywords: | Proteomics, continuous MS utilization, nanoLC-MS, Tandem nano-flow LC |
| Matrix: | cell lysate, HeLa protein digest |
| Author: | Christopher Pynn, Alexander Boychenko, Wim Decrop, Mike Baynham, Peter Jehle, Martin Ruehl |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
