Using ion chromatography methods to determine citrate and phosphate in pharmaceutical formulations has been previously demonstrated, validated, and adopted by The United States Pharmacopeia (USP) in General Chapter< 345>. The monograph specified an L61 column. Here we demonstrate that a higher porosity, higher capacity, and smaller particle size L81 column with similar selectivity to L61 has much higher efficiencies, capacities, and sensitivities, and extended linear range with reduced run times.

The determination of anions is one of the most important IC applications. Here we have updated Application Note 154 with the 4µm-resin-particle IonPac AS18-Fast-4µm column with expected backpressures approaching the previous system's limitations. In this Application Update, fast anion runs (9 min) were achieved with a gradient separation, saving 4 min run time with while retaining similar chromatography with higher sensitivity than the previous application. This analysis was facilitated by the high pressure capable Dionex Integrion HPIC system configured for Reagent-Free IC.

This record shows a 5-min, fast separation of anions in a municipal drinking water sample on the 4µm resin particle format IonPac AS22-Fast-4µm column. This method upgrades Application Brief AB120 by improving the separation using the 4µm resin particle column to produce more efficient peaks. The Integrion ion chromatography system is designed to work with the high pressures generated by the 4µm particles to deliver the improved separation.

The counter ion impacts the API drug’s efficacy, solubility, and toxicity, and therefore counter ion determinations are important to pharmaceutical companies during all stages of product development and as part of product quality monitoring. In this example, the sodium counter ion is determined in a water extract of a pharmaceutical drug tablet designed for pain relief. Here fast sodium determinations were demonstrated on an IonPac CS12A-8µm capillary column using a walk-up high pressure capillary IC system.

The counter ion impacts the API drug’s efficacy, solubility, and toxicity, and therefore counter ion determinations are important to pharmaceutical companies during all stages of product development and as part of product quality monitoring. In this example, the chloride counter ion is determined in a water extract of a pharmaceutical drug tablet designed to treat Type II diabetes patients. Here fast chloride determinations were demonstrated on an IonPac AS19 capillary column using a walk-up high pressure capillary IC system.