Hydrogen peroxide is an essential chemical in the fabrication of integrated circuit and microcircuit devices. Maximum allowable contaminate levels for semiconductor grade hydrogen peroxide can be as low as <100 ppt (ng/L) per individual inorganic cation. This application uses an IonPac CS17 column to determine trace cations and amines in hydrogen peroxide with a large-loop injection. The CS17 column separates amines without the organic solvent eluent modifier needed for separating amines when using older cation-exchange ion-chromatography (IC) columns.

Amines are found throughout nature and are synthesized for many commercial applications. They are used as pharmaceuticals, anti-corrosive agents, and chemical intermediates, and can indicate food spoilage. IC is an excellent technique for determining amines in a variety of samples, even low concentrations. Here is shown the separationand detection of two such amines: N,N-dimethyl-otoluidine and N,N-diethyl-p-toluidine. These amines are separated on a CS17 column with an MSA gradient and 9% acetonitrile, and are detected by absorbance at 210 nm.

This application note describes a cation-exchange chromatography method that significantly reduces the time between injections relative to the current USP method (by approximately 3 h) due to the very low hydrophobic character of the IonPac® CS17 column, enabling a faster elution of strongly retained compounds (e.g., cefepime). The linearity, detection limits, precision, and recovery of NMP in cefepime hydrochloride are determined. The limit of quantitation for this method is approximately 0.001% NMP, well within the limit of 0.3% set in the USP method.

A simple RFIC method is described for determination of carbachol, bethanechol, and choline in 25 min with a Dionex IonPac CS17 column. Methanesulfonic acid (MSA) eluent is delivered isocratically by an Eluent Generator (EG). Use of an EG eliminates eluent preparation errors and helps ensure retention time reproducibility. The sensitivity of suppressed conductivity detection allows carbachol determination in ophthalmic solutions with only a simple sample dilution.

三种胆碱类化合物在本实验条件下，可以被很好的分离和测定。此方法不需衍生，操作简单，且方法有很好的特异性和重现性，结果可靠，可用于卡巴胆碱注射液含量与有关物质的同时检测。